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KMID : 0545120010110050853
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Journal of Microbiology and Biotechnology
2001 Volume.11 No. 5 p.853 ~ p.857
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Flow Cytometric Analysis of the Effect of Silkworm Hemolymph on the Baculovirus-Induced Insect Cell Apoptosis
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Rhee Won-Jong
Park Tai-Hyun
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Abstract
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The effect of silkworm hemolymph on the inhibition of baculovirus-induced insect cell apoptosis was quantitatively investigated using a flow cytometric analysis. Spodoptera frugiperda (Sf9) cell and Autographa californica nuclear polyhedrous virus (AcNPV) were used as a model for insect cell and baculovirus in this study, respectively. Compared with a mammalian cell cycle, the fraction of G1 cells was relatively small in the Sf9 cell cycle. Silkworm hemolymph did not affect the Sf9 cell-cycle distribution before the baculovirus infection. However, the fraction of cells which are not in the sub-G1 phase remained at a high level for 3 days after the infection in the medium without silkworm hemolymph, while it remained at a high level for 7 days after the infection in the medium supplemented with silkworm hemolymph. The fractions of apoptotic cells in the sub-G1 phase were 4.7%, and 4 days after infection, 22.7%, in the media with and without silkworm hemolymph, respectively.
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KEYWORD
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